Avenanthramide C from germinated oats exhibits anti-allergic inflammatory effects in mast cells

Sci Rep. 2019 May 3;9(1):6884. doi: 10.1038/s41598-019-43412-2.


Mast cells play a crucial role in allergic diseases via the release of inflammatory mediators, particularly histamine and pro-inflammatory cytokines. Avenanthramide (Avn) C, a polyphenol found mainly in oats, is known to exhibit various biological properties. In this study, we aimed to evaluate the effectiveness of Avn C from germinated oats against mast cell-mediated allergic inflammation. For the in vitro study, RBL-2H3, mouse bone marrow-derived mast cells and rat peritoneal mast cells were used. Avn C (1-100 nM) inhibited the immunoglobulin (Ig)E-stimulated mast cells degranulation by suppressing phosphorylation of phosphoinositide 3-kinase and phospholipase Cγ1 and decreasing intracellular calcium levels. It inhibited IgE-stimulated secretion of inflammatory cytokines via suppression of FcεRI-mediated signaling proteins Lyn, Syk, Akt, and nuclear factor-κB. To verify the effects of Avn C in vivo, ovalbumin-induced active systemic anaphylaxis (ASA) and IgE-mediated passive cutaneous anaphylaxis (PCA) models were used. Oral administration of Avn C dose-dependently attenuated the ASA reactions, as evidenced by the inhibition of hypothermia and reduction of elevated serum histamine, IgE, and interleukin-4 levels. Avn C also inhibited the PCA reactions, such as ear swelling and plasma extravasation. Our results suggested that Avn C from germinated oats might be a possible therapeutic candidate for mast cell-mediated allergic inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Allergic Agents / pharmacology*
  • Anti-Inflammatory Agents / pharmacology*
  • Avena / chemistry*
  • Avena / growth & development*
  • Cell Degranulation / drug effects
  • Cytokines / metabolism
  • Germination*
  • Male
  • Mast Cells / drug effects*
  • Mast Cells / immunology
  • Mice
  • Mice, Inbred ICR
  • ortho-Aminobenzoates / pharmacology*


  • Anti-Allergic Agents
  • Anti-Inflammatory Agents
  • Cytokines
  • ortho-Aminobenzoates
  • avenanthramide-2C