Deciphering the Roles of N-Glycans on Collagen-Platelet Interactions

J Proteome Res. 2019 Jun 7;18(6):2467-2477. doi: 10.1021/acs.jproteome.9b00003. Epub 2019 May 15.

Abstract

Collagen is a potent agonist for platelet activation, presenting itself as a key contributor to coagulation via interactions with platelet glycoproteins. The fine details dictating platelet-collagen interactions are poorly understood. In particular, glycosylation could be a key determinant in the platelet-collagen interaction. Here, we report an affinity purification coupled to a mass spectrometry-based approach to elucidate the function of N-glycans in dictating platelet-collagen interactions. By integrative proteomic and glycoproteomic analysis of collagen-platelet interactive proteins with N-glycan manipulation, we demonstrate that the interaction of platelet adhesive receptors with collagen is highly N-glycan regulated, with glycans on many receptors playing positive roles in collagen binding, with glycans on other platelet glycoproteins exhibiting inhibitory roles on the binding to collagen. Our results significantly enhance our understanding of the details of glycans influencing the platelet-collagen interaction.

Keywords: N-glycans; affinity purification; collagen; collagen-platelet interactions; glycoproteins; glycoproteomics; intact glycopeptides; mass spectrometry; platelet; protein−protein interactions.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Sequence / genetics
  • Blood Platelets / metabolism*
  • Collagen / genetics
  • Collagen / metabolism*
  • Glycomics*
  • Glycoproteins / metabolism
  • Humans
  • Platelet Activation / genetics
  • Polysaccharides / genetics
  • Polysaccharides / metabolism*
  • Protein Binding / genetics
  • Protein Interaction Maps / genetics
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Glycoproteins
  • Polysaccharides
  • Collagen