PATZ1 is required for efficient HIV-1 infection

Biochem Biophys Res Commun. 2019 Jun 25;514(2):538-544. doi: 10.1016/j.bbrc.2019.04.175. Epub 2019 May 3.


Successful HIV-1 infection and subsequent replication deeply depend on how the virus usurps the host cell machinery. Identification and functional characterization of these host factors may represent a critical strategy for developing novel anti-HIV-1 therapy. Here, expression cloning with a cDNA expression library identified as an inhibitor of HIV-1 infection, a carboxy-terminally truncated form of human POZ/BTB and AT-hook- containing Zinc finger protein 1 (PATZ1), a transcriptional regulatory factor implicated in development and cancer. Knockdown or knockout of endogenous PATZ1 revealed a supportive role of PATZ1 in HIV-1 infection, but not in transduction with murine leukemia virus-based retroviral vector. More specifically, knockdown or knockout of PATZ1 impaired the viral cDNA synthesis but not the entry process and expression of two PATZ1 isoforms in PATZ1-KO cells restored susceptibility to HIV-1 infection. These results indicate that PATZ1 plays an important role in HIV-1 infection.

Keywords: HIV-1; Host factor; Infection; PATZ1; Reverse transcription; Virus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Gene Expression Regulation
  • Gene Library
  • HEK293 Cells
  • HIV-1 / genetics*
  • HIV-1 / metabolism
  • HIV-1 / pathogenicity
  • Host-Pathogen Interactions / genetics*
  • Humans
  • Kruppel-Like Transcription Factors / antagonists & inhibitors
  • Kruppel-Like Transcription Factors / genetics*
  • Kruppel-Like Transcription Factors / metabolism
  • Leukemia Virus, Murine / genetics
  • Leukemia Virus, Murine / metabolism
  • Lymphocytes / pathology
  • Lymphocytes / virology*
  • Mice
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / metabolism
  • RNA, Viral / biosynthesis
  • RNA, Viral / genetics*
  • Repressor Proteins / antagonists & inhibitors
  • Repressor Proteins / genetics*
  • Repressor Proteins / metabolism
  • Signal Transduction
  • Vesiculovirus / genetics
  • Vesiculovirus / metabolism


  • Kruppel-Like Transcription Factors
  • PATZ1 protein, human
  • RNA, Small Interfering
  • RNA, Viral
  • Repressor Proteins