Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay

Antimicrob Agents Chemother. 2019 Jun 24;63(7):e00550-19. doi: 10.1128/AAC.00550-19. Print 2019 Jul.

Abstract

We directly tested 484 organisms from clinical (n = 310) and simulated (n = 174) positive blood cultures using the NG-Test Carba 5 assay for carbapenemase-producing Enterobacterales detection. The assay identified all but 4 of the KPC (170/171), OXA-48-like (22/22), VIM (19/21), and NDM (14/15) producers with no false positives. Among the clinical Klebsiella pneumoniae organisms tested, 122 of 123 KPC, 1 of 1 OXA-48-like, and 1 of 2 VIM producers were detected by the assay. Some VIM and NDM producers yielded scant but still-readable bands with the assay. No organisms produced the IMPs that the assay was designed to detect.

Keywords: Enterobacterales; carbapenem resistance; immunochromatographic assay; positive blood culture.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / metabolism*
  • Bacteriological Techniques / methods*
  • Blood Culture / methods
  • Enterobacteriaceae / metabolism
  • Enterobacteriaceae Infections / diagnosis*
  • Enterobacteriaceae Infections / microbiology*
  • Humans
  • Klebsiella pneumoniae / metabolism
  • Microbial Sensitivity Tests / methods
  • Sensitivity and Specificity
  • beta-Lactamases / metabolism*

Substances

  • Bacterial Proteins
  • beta-Lactamases
  • carbapenemase