Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution

Nat Methods. 2019 Jun;16(6):501-504. doi: 10.1038/s41592-019-0401-3. Epub 2019 May 6.


We designed an epi-illumination SPIM system that uses a single objective and has a sample interface identical to that of an inverted fluorescence microscope with no additional reflection elements. It achieves subcellular resolution and single-molecule sensitivity, and is compatible with common biological sample holders, including multi-well plates. We demonstrated multicolor fast volumetric imaging, single-molecule localization microscopy, parallel imaging of 16 cell lines and parallel recording of cellular responses to perturbations.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Drosophila / metabolism*
  • HEK293 Cells
  • Humans
  • Image Processing, Computer-Assisted / methods*
  • Imaging, Three-Dimensional / methods*
  • Lighting / instrumentation*
  • Microscopy, Fluorescence / methods*
  • Molecular Imaging / methods*
  • Single-Cell Analysis / methods*
  • Spatio-Temporal Analysis