Expression of functional IL 2 receptors by lipopolysaccharide and interferon-gamma stimulated human monocytes

J Immunol. 1987 May 1;138(9):2917-22.

Abstract

Human peripheral blood monocytes were stimulated with lipopolysaccharide (LPS) and interferon-gamma (IFN-gamma) alone or in combination. Stimulated but not resting monocytes displayed the Tac peptide of the interleukin 2 (IL 2) receptor within 24 hr as measured by immunofluorescence staining and [3H] Tac binding. The total number of anti-Tac binding sites on co-stimulated monocytes was 13,700. By using scatchard analysis with radiolabeled IL 2, the activated cells were shown to express low numbers (below 100 sites/cell) of high affinity binding sites with a KD of approximately 15 pM. LPS and IFN-gamma were additive in augmenting the number of IL 2 and anti-Tac binding sites. By using an ELISA assay specific for the soluble released form of the Tac peptide we identified 112 U/ml of IL 2 receptors in the supernatant of monocytes stimulated for 24 hr with IFN-gamma, 233 U/ml after stimulation with LPS, and 519 U/ml after the addition of both stimulating agents. Both the membrane form (55,000 daltons), as well as the soluble form (45,000 to 50,000 daltons) of the Tac, IL 2 receptor, peptide from monocytes were shown by immunoprecipitation and gel electrophoresis to be similar size to the comparable forms of these receptors derived from activated T cells. In addition, monocytes stimulated for 8 hr contained mRNA specifically hybridizing to a cDNA probe coding for the Tac peptide. Finally, activated monocytes responded to the addition of recombinant IL 2 by an increase in H2O2 production that was measured by using fluorescent indicator 2,7-dichlorofluorescein. This response as well as the observed induction of monocytic IL 2 receptors by LPS may point to a functional role for this receptor during monocyte/macrophage responses to microbial infections.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle / drug effects
  • Gene Expression Regulation / drug effects
  • Humans
  • Hydrogen Peroxide / physiology
  • Interferon-gamma / pharmacology*
  • Interleukin-2 / physiology*
  • Lipopolysaccharides / pharmacology*
  • Monocytes / physiology*
  • RNA, Messenger / genetics
  • Receptors, Immunologic / physiology*
  • Receptors, Interleukin-2
  • Solubility

Substances

  • Interleukin-2
  • Lipopolysaccharides
  • RNA, Messenger
  • Receptors, Immunologic
  • Receptors, Interleukin-2
  • Interferon-gamma
  • Hydrogen Peroxide