Yersinia hibernica sp. nov., isolated from pig-production environments

Int J Syst Evol Microbiol. 2019 Jul;69(7):2023-2027. doi: 10.1099/ijsem.0.003422. Epub 2019 May 8.


A Gram-stain-negative, rod-shaped strain isolated from pig-production environments was identified as a new species within the genus Yersinia using multifaceted genomic and biochemical approaches. The genome of this strain was closed using a hybrid assembly approach combining both high accuracy short read sequencing data with long read sequencing technology. Phylogenetic analysis of the 16S rRNA gene showed ~98 % similarity to Yersinia kristensenii and ~98 % similarity to Yersinia enterocolitica. Average nucleotide identity (OrthoANI) values were calculated as 85.79 % to Y. kristensenii ATCC 33638T and 85.73 % to Y. enterocolitica ATCC 9610T thereby providing evidence that this isolate should be considered as a novel species. The type strain is CFS1934T (=NCTC 14222T=LMG 31076T).

Keywords: Illumina; Ireland; MinION; Oxford Nanopore Technologies; Yersinia.

MeSH terms

  • Animals
  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Ireland
  • Palatine Tonsil / microbiology
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Swine / microbiology*
  • Yersinia / classification*
  • Yersinia / isolation & purification


  • DNA, Bacterial
  • RNA, Ribosomal, 16S