Isolation and cross-sensitivity of X-ray-sensitive mutants of V79-4 hamster cells

Mutat Res. 1987 May;183(3):279-86. doi: 10.1016/0167-8817(87)90011-3.

Abstract

The V79-4 Chinese hamster line was mutagenized and surviving clones screened for X-ray sensitivity using a replica microwell technique. One slightly sensitive clone and 3 clearly sensitive clones were isolated from approximately 5000 screened, and designated irs 1 to irs 4. The 3 more sensitive clones showed different responses to the genotoxic agents mitomycin C (MMC), ethyl methanesulphonate (EMS) and ultraviolet light (UV). irs 1 showed considerable sensitivity to all the agents tested, in the order MMC much greater than EMS greater than UV. irs 2 and irs 3 had similar sensitivities to EMS and to UV (EMS greater than UV) but irs 3 was more sensitive than irs 2 to MMC. None of these mutants is identical in phenotype to previously published mutants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cell Survival / radiation effects
  • Cells, Cultured / radiation effects
  • Cricetinae
  • Cricetulus
  • DNA Repair
  • Ethyl Methanesulfonate / pharmacology
  • Ethylnitrosourea
  • Karyotyping
  • Mitomycin
  • Mitomycins / pharmacology
  • Mutation*
  • Ultraviolet Rays
  • X-Rays

Substances

  • Mitomycins
  • Mitomycin
  • Ethyl Methanesulfonate
  • Ethylnitrosourea