Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates

Front Mol Biosci. 2019 Apr 24:6:28. doi: 10.3389/fmolb.2019.00028. eCollection 2019.


A variant of 9°N DNA polymerase [Genbank ID (AAA88769.1)] with three mutations (D141A, E143A, A485L) and commercialized under the name "Therminator DNA polymerase" has the ability to incorporate a variety of modified nucleotide classes. This Review focuses on how Therminator DNA Polymerase has enabled new technologies in synthetic biology and DNA sequencing. In addition, we discuss mechanisms for increased modified nucleotide incorporation.

Keywords: DNA sequencing; aptamers; functionalized DNA; unnatural base pairs; xenonucleic acids.

Publication types

  • Review