The present study evaluated the microremediation potential of nine siderophore producing marine bacteria for hazardous raw tannery wastewater from common effluent treatment plant (CETP). Most of the pollutants detected in the wastewater were diminished after the bioremediation process. Further, among the three potent isolates selected for aerobic and anaerobic bioremediation study, Marinobacter hydrocarbonoclasticus demonstrated the highest bioremediation aerobically with a reduction in chromium (88%), sulphate (71%), phosphate (68%) and nitrate (57%). Notably, Nitratireductor kimnyeongensis could attack the effluent under both aerobic and anaerobic conditions as substantiated by statistically significant (p < .05) reduction in the pollutants [chromium (85%), sulphate (63%), Chemical Oxygen Demand (COD) (69%), phosphate (76%)]. From the study it is evident that the pollutant load reduction was achieved under both aerobic and anaerobic conditions, however, aerobic environment was more effective in reducing chromium, Biochemical Oxygen Demand (BOD), sulphate, nitrate and phosphate. The bioremediation efficiency was further confirmed by the bioassay experiments with plant and animal models where higher seed germination, greater plant length and biomass, as well as improved survival rate of Artemia nauplii for bioremediated wastewater was observed as compared to the untreated effluent indicating a significant reduction in toxicity. The results for simultaneous removal of multiple-toxicants thus signify effectiveness and ease of using the robust properties of these marine bacterial strains suggesting their potential application for bioremediation. Hence this could pave a promising way for an environment-friendly and economically feasible clean-up strategy for safer disposal of tannery wastewater.
Keywords: Bioremediation; marine bacteria; multiple-toxicants; tannery; toxicity bioassay.