Extraction of starch by dimethyl sulfoxide and quantitation by enzymatic assay

Anal Biochem. 1987 Feb 15;161(1):132-9. doi: 10.1016/0003-2697(87)90662-2.

Abstract

A method for the rapid, sensitive, and specific determination of starch in plant tissues is described. Starch from a variety of plant tissues is solubilized by stirring for 24 h or by sonication for 40 min in dimethyl sulfoxide. Dilution of this extract to less than 20% dimethyl sulfoxide permits a nearly complete hydrolysis of the starch in less than 3 h with glucoamylase from Rhizopus niveus. Quantitation of liberated glucose by a coupled hexokinase and glucose-6-phosphate dehydrogenase method provides an additional degree of specificity.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amylopectin / analysis
  • Amylose / analysis
  • Dimethyl Sulfoxide*
  • Glucan 1,4-alpha-Glucosidase
  • Glucose / analysis
  • Glucosephosphate Dehydrogenase
  • Hexokinase
  • Hydrolysis
  • Plants / analysis
  • Starch / analysis*

Substances

  • Starch
  • Amylose
  • Amylopectin
  • Glucosephosphate Dehydrogenase
  • Hexokinase
  • Glucan 1,4-alpha-Glucosidase
  • Glucose
  • Dimethyl Sulfoxide