Characterization of new variant human ES line VH9 hESC (INSTEMe001-a): a tool for human stem cell and cancer research

Stem Cell Res. 2019 May;37:101444. doi: 10.1016/j.scr.2019.101444. Epub 2019 Apr 18.


Human pluripotent stem cells (hPSCs) acquire changes at the genomic level upon proliferation and differentiation (Peterson and Loring, 2014). Studies from International Stem Cell Initiative and independent laboratories identified a copy number variant (CNV) in hES cell lines displaying a normal karyotype, which provided a selective advantage to hES cells in culture. In our laboratory we have identified variant H9-hESC (derived from H9-hESC) with normal karyotype, pluripotency expression, differentiation profile but with altered traits of high cell survival and low E-CADHERIN expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomedical Research / statistics & numerical data*
  • Cell Culture Techniques / methods*
  • Cell Differentiation*
  • Cells, Cultured
  • Embryonic Stem Cells / cytology*
  • Humans
  • Mice
  • Mice, Inbred NOD
  • Mice, SCID
  • Pluripotent Stem Cells / cytology*
  • Teratoma / pathology*