CRISPR/Cas Powered Multiplexed Biosensing

Trends Biotechnol. 2019 Aug;37(8):791-792. doi: 10.1016/j.tibtech.2019.04.005. Epub 2019 May 8.


Multiplexed CRISPR/Cas biosensing offers various possibilities for diagnosing different diseases by analyzing many analytes from one single specimen. However, in this relatively new field, nearly no multiplexing approaches exist, as many challenges need to be addressed. We discuss the reasons behind it and possible strategies to push the multiplexing level.

Keywords: CRISPR/Cas systems; multiplexing; nucleic acid diagnostics; point-of-care testing.

Publication types

  • Letter

MeSH terms

  • Biosensing Techniques / methods*
  • Biosensing Techniques / trends
  • CRISPR-Cas Systems*
  • Diagnostic Tests, Routine / methods*
  • Diagnostic Tests, Routine / trends
  • Humans
  • Molecular Diagnostic Techniques / methods*
  • Molecular Diagnostic Techniques / trends