CRISPR/Cas Powered Multiplexed Biosensing

Richard Bruch; Gerald A Urban; Can Dincer

doi:10.1016/j.tibtech.2019.04.005

CRISPR/Cas Powered Multiplexed Biosensing

Trends Biotechnol. 2019 Aug;37(8):791-792. doi: 10.1016/j.tibtech.2019.04.005. Epub 2019 May 8.

Authors

Richard Bruch¹, Gerald A Urban², Can Dincer³

Affiliations

¹ University of Freiburg, Department of Microsystems Engineering (IMTEK), Laboratory for Sensors, 79110 Freiburg, Germany; University of Freiburg, Freiburg Center for Interactive Materials and Bioinspired Technologies (FIT), 79110 Freiburg, Germany.
² University of Freiburg, Department of Microsystems Engineering (IMTEK), Laboratory for Sensors, 79110 Freiburg, Germany; University of Freiburg, Freiburg Materials Research Center (FMF), 79104 Freiburg, Germany.
³ University of Freiburg, Department of Microsystems Engineering (IMTEK), Laboratory for Sensors, 79110 Freiburg, Germany; University of Freiburg, Freiburg Center for Interactive Materials and Bioinspired Technologies (FIT), 79110 Freiburg, Germany; Imperial College London, Department of Bioengineering, Royal School of Mines, SW7 2AZ, London, UK. Electronic address: dincer@imtek.de.

PMID: 31078316
DOI: 10.1016/j.tibtech.2019.04.005

Abstract

Multiplexed CRISPR/Cas biosensing offers various possibilities for diagnosing different diseases by analyzing many analytes from one single specimen. However, in this relatively new field, nearly no multiplexing approaches exist, as many challenges need to be addressed. We discuss the reasons behind it and possible strategies to push the multiplexing level.

Keywords: CRISPR/Cas systems; multiplexing; nucleic acid diagnostics; point-of-care testing.

Publication types

Letter

MeSH terms

Biosensing Techniques / methods*
Biosensing Techniques / trends
CRISPR-Cas Systems*
Diagnostic Tests, Routine / methods*
Diagnostic Tests, Routine / trends
Humans
Molecular Diagnostic Techniques / methods*
Molecular Diagnostic Techniques / trends