Development of a loop-mediated isothermal amplification assay for Vibrio cholerae O1 and O139

Mol Cell Probes. 2019 Jun;45:65-67. doi: 10.1016/j.mcp.2019.05.001. Epub 2019 May 10.

Abstract

A loop-mediated isothermal amplification assay was developed. It was designed for recognizing Vibrio cholerae O1/O139, where atpA, rfbN, and wfbR genes were adopted. The assay specifically detected the target with sensitivities of 5-67 copies per reaction in 1 h. The assay will aid rapid detection of the cholera bacterium.

Keywords: Cholera; LAMP; O1/O139.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics*
  • Cholera / diagnosis
  • Environmental Monitoring
  • Humans
  • Limit of Detection
  • Molecular Diagnostic Techniques / methods*
  • Nucleic Acid Amplification Techniques / methods*
  • Vibrio cholerae O1 / genetics
  • Vibrio cholerae O1 / isolation & purification*
  • Vibrio cholerae O139 / genetics
  • Vibrio cholerae O139 / isolation & purification*

Substances

  • Bacterial Proteins
  • RfbN protein, Vibrio cholerae

Supplementary concepts

  • LAMP assay