Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits

Miljana Baćević; Božidar Brković; France Lambert; Ljiljana Djukić; Nina Petrović; Jelena Roganović

doi:10.1016/j.archoralbio.2019.05.005

Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits

Arch Oral Biol. 2019 Jun:102:231-237. doi: 10.1016/j.archoralbio.2019.05.005. Epub 2019 May 6.

Authors

Miljana Baćević¹, Božidar Brković², France Lambert³, Ljiljana Djukić⁴, Nina Petrović⁵, Jelena Roganović⁶

Affiliations

¹ Dental Biomaterial Research Unit (d-BRU), Faculty of Medicine, University of Liege, Liege, Belgium.
² Department of Oral Surgery, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.
³ Department of Periodontology and Oral Surgery, Faculty of Medicine, University of Liege, Belgium.
⁴ Department of Pharmacology in Dentistry, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.
⁵ Department of Radiobiology and Molecular Genetics, Institute of Nuclear Sciences Vinča, University of Belgrade, Belgrade, Serbia; Institute of Oncology and Radiology of Serbia, Belgrade, Serbia.
⁶ Department of Pharmacology in Dentistry, School of Dental Medicine, University of Belgrade, Belgrade, Serbia. Electronic address: jelena.roganovic@stomf.bg.ac.rs.

PMID: 31082699
DOI: 10.1016/j.archoralbio.2019.05.005

Abstract

Objective: Leukocyte- and platelet-rich fibrin (L-PRF) represents a natural, low-cost product which may promote tissue healing by mechanisms not fully elucidated. Diabetes mellitus (DM) disrupts bone healing by inducing inflammation and oxidative stress (OS), mechanisms regulated by microRNAs (miRs). The aim of the present study was to investigate the microRNA-21 (miR-21) involvement in diabetic bone regeneration using L-PRF alone or in combination with a standard grafting material.

Design: After the induction of diabetes (alloxan 100 mg/kg), four cranial osteotomies were made in diabetic (n = 12) and non-diabetic (n = 12) rabbits: one was left empty and the remaining three were grafted with L-PRF, bovine hydroxyapatite (Bio-Oss^®) and L-PRF + Bio-Oss®. Two and eight weeks postoperatively, the samples were harvested for miR-21 expression (Real-time RT-PCR) and enzyme-linked immunosorbent assay analyses.

Results: Diabetic rabbits showed decreased miR-21 and matrix metalloproteinase-9 (MMP-9) protein expression while increased malondialdehyde (MDA) levels two weeks postoperatively; however, there were no significant differences in miR-21 and MMP-9 levels between diabetic and non-diabetic rabbits in samples taken eight weeks postoperatively. Application of L-PRF and L-PRF + Bio-Oss^® improved miR-21 and MMP-9 and decreased MDA levels while Bio-Oss® alone enhanced superoxide dismutase (SOD) activity levels in diabetic rabbits.

Conclusion: L-PRF alone or in combination with bovine hydroxyapatite as bone graft could be beneficial in DM since it seems to improve inflammation-modulatory miR-21 expression and decreases oxidative stress.

Keywords: Bone healing; Diabetes; L-PRF; MMP-9; Oxidative stress; microRNA-21.

MeSH terms

Animals
Blood Platelets
Cattle
Diabetes Mellitus*
Fibrin
MicroRNAs
Oxidative Stress*
Platelet-Rich Fibrin*
Rabbits

Substances

MicroRNAs
Fibrin