Enzymatic Hydrolysis of a Collagen Hydrolysate Enhances Postprandial Absorption Rate-A Randomized Controlled Trial

Nutrients. 2019 May 13;11(5):1064. doi: 10.3390/nu11051064.


Collagen is characterized by its high content of glycine, proline and hydroxyproline, and is found to exert beneficial effects on joint pain related to activity and osteoarthritis. However, to exert any beneficial effects it is essential that collagen is optimally absorbed. This study aimed to investigate the postprandial absorption of collagen and elucidate the impact of an exogenous enzymatic hydrolysis on absorption rate and bioavailability. A randomized, blinded, cross-over study was conducted where ten healthy male subjects received either 35 g enzymatically hydrolyzed collagen protein (EHC), 35 g non-enzymatically hydrolyzed collagen protein (NC) or placebo (250 mL water) on three nonconsecutive days. Blood samples were drawn before, and up to 240 min following, ingestion and the blood metabolome was characterized by nuclear magnetic resonance (NMR)-based metabolomics. A significant increase in the plasma concentration of nearly all amino acids (AAs) was observed over a 240 min period for both EHC and NC. In addition, the absorption rate and bioavailability of glycine, proline and hydroxyproline were significantly higher for EHC (p < 0.05). In conclusion, ingestion of collagen hydrolysates increases postprandial plasma concentrations of AAs over a period of 240 min, and an enzymatic hydrolysis increases the absorption rate and bioavailability of the collagen-rich AAs glycine, proline and hydroxyproline.

Keywords: amino acid absorption; collagen absorption; collagen nutriceuticals; collagen uptake; dietary protein; glycine; hydroxyproline; nutrimetabolomics; proline; sports nutrition.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Amino Acids / blood
  • Amino Acids / metabolism
  • Blood Glucose
  • Collagen / chemistry*
  • Collagen / metabolism*
  • Cross-Over Studies
  • Humans
  • Hydrolysis
  • Male
  • Postprandial Period / physiology*
  • Protein Hydrolysates / chemistry*
  • Protein Hydrolysates / metabolism*


  • Amino Acids
  • Blood Glucose
  • Protein Hydrolysates
  • Collagen