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The Effect of Hyperlipidemia on Bone Graft Regeneration of Peri-Implantal Created Defects in Rabbits


The Effect of Hyperlipidemia on Bone Graft Regeneration of Peri-Implantal Created Defects in Rabbits

Mehmet Bugrul Tekin et al. Int J Implant Dent.


Aim: It is reported that hyperlipidemia affects quality and density of bone and adversely affects wound healing. The effect of hyperlipidemia on implant osseointegration and peri-implant defect regeneration has not been fully explained. The purpose of this study was to examine the effects of hyperlipidemia on the healing potential of the materials used for peri-implant bone regeneration and implant stability.

Materials and methods: Twelve male, New Zealand rabbits were used in this study. Half of the rabbits were fed a 2% cholesterol diet for 8 weeks to induce hypercholesterolemia. Peri-implant defects (7 mm diameter) were created in the tibias of rabbits and placed implants (3.3 mm in diameter). This study was conducted as a split-mouth design. Animals were randomly divided into two groups: (1) hypercholesterol+autogenous graft group and hypercholesterol+xenograft group (n = 6), and (2) autogenous graft and xenograft groups as controls (n = 6). At 8 weeks after surgery, the rabbits were euthanized. During implant surgery and at 8 weeks, implant stability was measured with resonance frequency analysis (RFA values). Bone-to-implant contact (BIC) was analyzed via histomorphometric analysis.

Results: Hyperlipidemic groups showed significantly lower BIC values than those of the control groups at 8 weeks (p < 0.05). According to baseline RFA readings, there was no significant difference between control and hyperlipidemic groups (p ˃ 0.05). The hypercholesterol+autogenous graft group had significantly lower RFA readings and BIC values than the hypercholesterol+xenograft group at 8 weeks (p < 0.05).

Conclusion: Within the limitations of this study, it was found that hyperlipidemia may negatively affect the implant stability especially in the autogenous group and also, may decrease peri-implant bone regeneration. However, further studies are necessary to confirm these results more.

Keywords: Bone regeneration; Hyperlipidemia; Implant stability; Peri-implant.

Conflict of interest statement

Ethics approval and consent to participate

This protocol was approved by the Animal Ethics Committee of Cumhuriyet University Faculty of Medicine under protocol number 65202830/130.

Consent for publication

Not applicable

Competing interests

Mehmet Bugrul Tekin and Hulya Toker declare that they have no competing interests.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.


Fig. 1
Fig. 1
Mean BIC value of study groups at 8 weeks. *p < 0.05 versus HPL+AG and HPL+XG group, #p < 0.05 versus HPL+XG group (n of each group is 5)
Fig. 2
Fig. 2
Histologic views of the study groups at 8 weeks (toluidine blue, × 10). a AG group, b XG group, c HPL+AG group, d HPL+XG group (NB new bone, BM bone marrow, red arrow indicates bone-to-implant contact, black arrow indicates residual xenograft particles)
Fig. 3
Fig. 3
Mean and standard deviation of RFA readings in study groups at baseline and 8 weeks. ap < 0.05 versus baseline, bp < 0.05 versus HPL+AG group, cp < 0.05 versus AG group (n of each group is 5)

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