[Protein Biosynthesis Proofreading Is Closely Associated with the Existence of Factor-Free Ribosomal Synthesis]

Mol Biol (Mosk). 2019 Mar-Apr;53(2):349-352. doi: 10.1134/S0026898419020046.
[Article in Russian]


Despite protein biosynthesis being studied for decades, some major questions concerning this process are still to be addressed. We elucidate a close connection between proofreading of the emerging amino acid sequence during its normal, elongation factor-dependent ribosomal biosynthesis and the existence of the factor-free synthesis of a polypeptide chain on a ribosome. In this factor-free process, the biological role of proofreading is played by a process opposite to the factor-free attachment of Aa-tRNA to the ribosome, namely, the removal via the same pathway of that Aa-tRNA, which is not complementary to the mRNA codon exhibited by the ribosome.

Keywords: GTP; biosynthesis of polypeptides; codon recognition; elongation factor Tu; factor-free process; free energy; parallel reactions; proofreading; ribosome.

MeSH terms

  • Codon / genetics
  • Protein Biosynthesis*
  • RNA, Messenger / genetics
  • RNA, Transfer / genetics
  • RNA, Transfer / metabolism
  • Ribosomes / metabolism*
  • Thermodynamics


  • Codon
  • RNA, Messenger
  • RNA, Transfer