A DNA Aptamer for Cyclic Adenosine Monophosphate that Shows Adaptive Recognition

Chembiochem. 2020 Jan 15;21(1-2):157-162. doi: 10.1002/cbic.201900259. Epub 2019 Aug 29.

Abstract

As a ubiquitous second messenger, cyclic adenosine monophosphate (cAMP) mediates diverse biological processes such as cell growth, inflammation, and metabolism. The ability to probe these pathways would be significantly enhanced if we had a DNA-based sensor for cAMP. Herein, we describe a new, 31-base long single-stranded DNA aptamer for cAMP, denoted caDNApt-1, that was isolated by in vitro selection using systemic evolution of ligands after exponential enrichment (SELEX). caDNApt-1 has an approximately threefold higher affinity for cAMP than ATP, ADP, and AMP. Using non-denaturing gel electrophoresis and fluorescence spectroscopy, we characterized the structural changes caDNApt-1 undergoes upon binding to cAMP and revealed its potential as a cAMP sensor.

Keywords: G-quadruplexes; adaptive recognition; aptamers; cyclic adenosine monophosphate; nucleic acids.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide / chemistry*
  • Cyclic AMP / analysis*
  • Nucleic Acid Conformation
  • SELEX Aptamer Technique*

Substances

  • Aptamers, Nucleotide
  • Cyclic AMP