The beneficial effects of 15 units of high-intensity circuit training in women is modified by age, baseline insulin resistance and physical capacity

Diabetes Res Clin Pract. 2019 Jun;152:156-165. doi: 10.1016/j.diabres.2019.05.009. Epub 2019 May 15.

Abstract

Aim: To investigate the effect of a single and 15 units of high-intensity circuit training (HICT) programme on glucose metabolism, myokines' response and selected genes' expression in women.

Methods: Thirty-three, non-active women (mean age: 38 ± 12) were split into a HICT (n = 20) or a control group (CON, n = 13). The training protocol included three circuits of nine exercises with own body weight as a workload performed 3 times a week for five weeks. The CON group performed HICT twice. Blood samples were taken before, 1 h and 24 h after the first and last unit to determine IGF-1, myostatin, irisin, decorin, HSP27, interleukin-15 concentrations using the ELISA immunoenzymatic method. To evaluate HSPB1, TNF-α and DCN mRNA, real-time PCR was used. Pre- and post-intervention, the oral glucose test and body composition assessment were completed.

Results: The following parameters tended to decrease after the 5-week HICT program: insulin and HOMA-IR Training diminished insulin/IGF-1 ratio (51% CI: -63% to -34%) and induced the drop of myostatin concentration but significantly only among middle-aged women and at baseline insulin resistance.

Conclusion: Obtained data revealed that HICT improved an insulin sensitivity and diminished myostatin concentration among older, insulin-resistant women with lower baseline physical capacity.

Keywords: Decorin; Heat shock protein; Insulin growth factors; Insulin resistance; Oral glucose tolerance test.

Publication types

  • Controlled Clinical Trial

MeSH terms

  • Adult
  • Age Factors
  • Aging / physiology*
  • Blood Glucose / metabolism
  • Body Composition / physiology
  • Circuit-Based Exercise* / methods
  • Decorin / genetics
  • Decorin / metabolism
  • Energy Metabolism / genetics
  • Exercise / physiology
  • Exercise Therapy / methods*
  • Exercise Tolerance / physiology*
  • Female
  • Gene Expression Regulation
  • Heat-Shock Proteins / genetics
  • Heat-Shock Proteins / metabolism
  • Humans
  • Insulin / blood
  • Insulin Resistance / physiology*
  • Insulin-Like Growth Factor I / genetics
  • Insulin-Like Growth Factor I / metabolism
  • Middle Aged
  • Myostatin / genetics
  • Myostatin / metabolism
  • Physical Fitness / physiology*
  • Resistance Training / methods
  • Young Adult

Substances

  • Blood Glucose
  • DCN protein, human
  • Decorin
  • Heat-Shock Proteins
  • IGF1 protein, human
  • Insulin
  • MSTN protein, human
  • Myostatin
  • Insulin-Like Growth Factor I