MiR-202 inhibits cell proliferation, invasion, and migration in breast cancer by targeting ROCK1 gene

J Cell Biochem. 2019 Sep;120(9):16008-16018. doi: 10.1002/jcb.28879. Epub 2019 May 20.


Purpose: The aim of this study was to research the effect of miR-202 on breast cancer cells proliferation, invasion, and migration.

Methods: TCGA analysis was used to research miR-202 expression and overall survival in patients with breast cancer. Transfection with miR-202 mimics or cotransfection with miR-202 mimics and ROCK1 expression vector was performed on breast cancer cells. Reverse transcription polymerase chain reaction was used to detect the miR-202 expression of breast cancer tissues and cells. Western blot was conducted to research the expression of ROCK1, E-cadherin, Twist, N-cadherin, and MMP2. Dual luciferase reporter assay was performed to detect the targeted relationship between ROCK1 and miR-202. MTT and transwell assay were used to detect breast cancer cells proliferation, invasion, and migration.

Results: Downregulation of miR-202 was positively correlated with poor prognosis of patients with breast cancer. miR-202 in breast cancer tissues and breast cancer cells was significantly downregulated. Upregulation of miR-202 inhibited the proliferation, migration, and invasion of breast cancer cells. miR-202 promoted E-cadherin expression and inhibited the expression of N-cadherin, Twist, and MMP2. ROCK1 was the target gene of miR-202. miR-202 regulated proliferation, migration, invasion, and expression of related proteins in breast cancer cells by targeting ROCK1 expression.

Conclusions: miR-202 inhibited breast cancer cells proliferation, invasion, and migration by targeting the ROCK1 gene.

Keywords: breast cancer; invasion; miR-202; migration; proliferation.

MeSH terms

  • 3' Untranslated Regions
  • Breast Neoplasms / genetics*
  • Cell Line, Tumor
  • Cell Movement
  • Cell Proliferation
  • Down-Regulation*
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • MCF-7 Cells
  • MicroRNAs / genetics*
  • Neoplasm Invasiveness
  • Prognosis
  • rho-Associated Kinases / genetics*


  • 3' Untranslated Regions
  • MIRN202 microRNA, human
  • MicroRNAs
  • ROCK1 protein, human
  • rho-Associated Kinases