Abstract
This study was to compare the effects of parenteral supplementation of methionyl-methionine (Met-Met) or Met on intestinal barrier function in Met-deficient pregnant mice. Pregnant mice were randomly divided into three groups. The Control group was provided a diet containing Met and received i.p. injection of saline. The Met group was fed the same diet but without Met and received daily i.p. injection of 35% of the Met contained in the control diet. The Met-Met group was treated the same as the Met group, except that 25% of the Met injected was replaced with Met-Met. Met-Met promoted villus surface area in ileum compared with Met alone. In addition, the mRNA abundance of amino acid and glucose transporters in the small intestine was altered with Met-Met. Moreover, Met-Met increased tight junction protein and decreased apoptosis-related proteins expression in the jejunum and ileum. These results suggest that Met-Met can promote intestinal function over Met alone in Met-deficient mice.
Keywords:
apoptosis; intestinal function; methionine; methionyl-methionine; tight junction.
© 2019 Blackwell Verlag GmbH.
MeSH terms
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Amino Acid Transport Systems
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Animals
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Apoptosis / drug effects
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Apoptosis / physiology
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Dipeptides / administration & dosage
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Dipeptides / pharmacology*
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Female
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Gene Expression Regulation / drug effects
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Glucose Transport Proteins, Facilitative / genetics
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Glucose Transport Proteins, Facilitative / metabolism
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Intestines / drug effects*
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Methionine / administration & dosage
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Methionine / deficiency
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Methionine / pharmacology*
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Mice
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Peptide Transporter 1 / genetics
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Peptide Transporter 1 / metabolism
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Pregnancy
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Random Allocation
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Tight Junction Proteins / genetics
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Tight Junction Proteins / metabolism
Substances
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Amino Acid Transport Systems
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Dipeptides
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Glucose Transport Proteins, Facilitative
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Peptide Transporter 1
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Slc15a1 protein, mouse
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Tight Junction Proteins
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methionylmethionine
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Methionine
Associated data
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GENBANK/NM_175121.4
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GENBANK/NM_009201.2
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GENBANK/NM_001359603.1
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GENBANK/NM_020049.4
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GENBANK/NM_001199015.1
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GENBANK/NM_139142.2
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GENBANK/NM_011404.3
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GENBANK/NM_016972.2
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GENBANK/NM_001199284.1
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GENBANK/NM_153139.4
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GENBANK/NM_028247.4
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GENBANK/NM_009199.2
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GENBANK/NM_011990.2
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GENBANK/NM_001301424.1
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GENBANK/NM_001253680.1
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GENBANK/NM_001357381.1
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GENBANK/NM_019810.4
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GENBANK/NM_019741.3
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GENBANK/NM_011149.2
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GENBANK/NM_007475.5
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GENBANK/NM_007393.5