Direct comparison of chitinolytic properties and determination of combinatory effects of mouse chitotriosidase and acidic mammalian chitinase

Int J Biol Macromol. 2019 Aug 1;134:882-890. doi: 10.1016/j.ijbiomac.2019.05.097. Epub 2019 May 17.

Abstract

Chitotriosidase (Chit1) and acidic mammalian chitinase (AMCase) have been implicated in food processing and various pathophysiological conditions such as chronic inflammatory diseases. By combination of the colorimetric analysis and fluorophore-assisted carbohydrate electrophoresis (FACE) method, we directly compared the chitinolytic properties of mouse Chit1 and AMCase and determined their combinatory effects in artificial and natural chitin substrates processing. Chit1 and AMCase display different dynamics of chitinolytic properties through acidic to neutral conditions. At pH2.0, the activity of AMCase was higher than that of Chit1 and stronger or comparable with that of Serratia marcescens chitinase B, a well-characterized bacterium chitinase. Changes of degradation products using different substrates indicate that AMCase and Chit1 have diverse properties under various pH conditions. Exposure of the chitin substrates to both Chit1 and AMCase did not indicate any mutual interference of these enzymes and showed no synergistic effect, in contrast to observations regarding some bacterial chitinases. Our results suggest that Chit1 and AMCase have no synergistic effect under physiological conditions.

Keywords: Acidic mammalian chitinase; Chitin substrates; Chitotriosidase; Direct comparison; Mutual effects; Serratia marcescens chitinase B.

MeSH terms

  • Animals
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics
  • Chitin / chemistry*
  • Chitinases / chemistry*
  • Chitinases / genetics
  • Colorimetry
  • Hexosaminidases / chemistry*
  • Hydrogen-Ion Concentration
  • Hydrolysis
  • Mice
  • Molecular Weight
  • Recombinant Proteins
  • Substrate Specificity

Substances

  • Bacterial Proteins
  • Recombinant Proteins
  • Chitin
  • Hexosaminidases
  • chitotriosidase
  • Chitinases
  • chitinase B, Serratia marcescens