LncRNA NR2F1-AS1 is involved in the progression of endometrial cancer by sponging miR-363 to target SOX4

Pharmazie. 2019 May 1;74(5):295-300. doi: 10.1691/ph.2019.8905.

Abstract

This study intended to investigate the role of lncRNA NR2F1-AS1 in endometrial cancer (EC). The expression level of NR2F1-AS1 in tumor tissues and EC cells was measured. After sh-NR2F1-AS1 transfection, the cell viability, apoptosis, migration and invasion of EC cells were analyzed. Luciferase reporter assay was conducted to investigate the target gene of miR-363. The expression levels of PI3K/AKT/GSK-3β pathway-associated factors were assayed using western blot. NR2F1-AS1 was significantly overexpressed in EC tissues and cells. NR2F1-AS1 inhibition decreased EC cell viability, migration and invasion, while promoted cell apoptosis. miR-363 was negatively regulated by NR2F1-AS1. SOX4 was a target of miR-363. NR2F1-AS1 functioned on EC progression via PI3K/AKT/GSK-3β pathway. The results demonstrated that NR2F1-AS1 was highly expressed in EC, which involved in the proliferation and migration of EC cells through downregulation of miR-363 to target SOX4 and regulating PI3K/AKT/GSK-3β pathway.

MeSH terms

  • Adult
  • Aged
  • Apoptosis / physiology
  • COUP Transcription Factor I / biosynthesis
  • COUP Transcription Factor I / genetics*
  • Cell Line, Tumor
  • Cell Movement / physiology
  • Cell Proliferation / physiology
  • Disease Progression
  • Endometrial Neoplasms / genetics
  • Endometrial Neoplasms / metabolism*
  • Endometrial Neoplasms / pathology
  • Female
  • Glycogen Synthase Kinase 3 / metabolism
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Middle Aged
  • Neoplasm Invasiveness
  • Neoplasm Metastasis
  • Phosphatidylinositol 3-Kinases / metabolism
  • RNA, Antisense / genetics
  • RNA, Antisense / metabolism*
  • RNA, Long Noncoding / genetics
  • RNA, Long Noncoding / metabolism*
  • SOXC Transcription Factors / genetics
  • SOXC Transcription Factors / metabolism*
  • Signal Transduction

Substances

  • COUP Transcription Factor I
  • MIRN363 microRNA, human
  • MicroRNAs
  • NR2F1 protein, human
  • RNA, Antisense
  • RNA, Long Noncoding
  • SOX4 protein, human
  • SOXC Transcription Factors
  • Glycogen Synthase Kinase 3