Lotus (Nelumbo nucifera) seed protein isolate exerts anti-inflammatory and antioxidant effects in LPS-stimulated RAW264.7 macrophages via inhibiting NF-κB and MAPK pathways, and upregulating catalase activity

Int J Biol Macromol. 2019 Aug 1:134:791-797. doi: 10.1016/j.ijbiomac.2019.05.094. Epub 2019 May 18.

Abstract

Lotus seed has long been used in traditional medicine and cuisine. In this study, lotus seed protein (LSP) was isolated and evaluated its anti-inflammatory effect in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. LSP isolate (LSPI) treatment in LPS-stimulated RAW264.7 macrophages resulted in the significant (p < 0.05) decrease of NO production by downregulation of the expressions of mRNA and protein, including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). In addition, LSPI treatment attenuated the production of reactive oxygen species (ROS) through increasing catalase activity, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-1β in LPS-stimulated RAW264.7 macrophages. Furthermore, LPS stimulation in RAW264.7 macrophages caused the translocation of nuclear factor-kappa B (NF-κB) into the nucleus and the phosphorylation of mitogen-activated protein kinase (MAPK) but these stimulations were abolished by LSPI treatment.

Keywords: Anti-inflammation; MAPK; NF-κB; Nelumbo nucifera; Protein; RAW264.7 macrophages.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / chemistry
  • Anti-Inflammatory Agents / pharmacology*
  • Antioxidants / chemistry
  • Antioxidants / pharmacology*
  • Cell Survival / drug effects
  • Cytokines / metabolism
  • Gene Expression Regulation, Plant / drug effects
  • Inflammation Mediators / metabolism
  • Lipopolysaccharides / immunology
  • Lotus / chemistry*
  • Macrophage Activation / drug effects
  • Macrophage Activation / immunology
  • Macrophages / drug effects*
  • Macrophages / physiology*
  • Mice
  • Mitogen-Activated Protein Kinases
  • NF-kappa B / metabolism
  • Phosphorylation
  • Plant Proteins / chemistry
  • Plant Proteins / pharmacology*
  • RAW 264.7 Cells
  • Reactive Oxygen Species / metabolism
  • Seeds / chemistry*
  • Signal Transduction / drug effects*

Substances

  • Anti-Inflammatory Agents
  • Antioxidants
  • Cytokines
  • Inflammation Mediators
  • Lipopolysaccharides
  • NF-kappa B
  • Plant Proteins
  • Reactive Oxygen Species
  • Mitogen-Activated Protein Kinases