MetaDome: Pathogenicity analysis of genetic variants through aggregation of homologous human protein domains

Hum Mutat. 2019 Aug;40(8):1030-1038. doi: 10.1002/humu.23798. Epub 2019 Jun 18.


The growing availability of human genetic variation has given rise to novel methods of measuring genetic tolerance that better interpret variants of unknown significance. We recently developed a concept based on protein domain homology in the human genome to improve variant interpretation. For this purpose, we mapped population variation from the Exome Aggregation Consortium (ExAC) and pathogenic mutations from the Human Gene Mutation Database (HGMD) onto Pfam protein domains. The aggregation of these variation data across homologous domains into meta-domains allowed us to generate amino acid resolution of genetic intolerance profiles for human protein domains. Here, we developed MetaDome, a fast and easy-to-use web server that visualizes meta-domain information and gene-wide profiles of genetic tolerance. We updated the underlying data of MetaDome to contain information from 56,319 human transcripts, 71,419 protein domains, 12,164,292 genetic variants from gnomAD, and 34,076 pathogenic mutations from ClinVar. MetaDome allows researchers to easily investigate their variants of interest for the presence or absence of variation at corresponding positions within homologous domains. We illustrate the added value of MetaDome by an example that highlights how it may help in the interpretation of variants of unknown significance. The MetaDome web server is freely accessible at

Keywords: ClinVar; Pfam; genetic tolerance; genetic variation; gnomAD; meta-domains; pathogenicity; protein domain homology; web server.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Computational Biology / methods*
  • Databases, Genetic
  • Genetic Predisposition to Disease
  • Genetic Variation*
  • Genome, Human
  • Humans
  • Internet
  • Protein Domains
  • Proteins / chemistry*
  • Proteins / genetics*
  • Software
  • Structural Homology, Protein


  • Proteins