Inhibition of endocytosis by anti-clathrin antibodies

Cell. 1987 Jul 31;50(3):453-63. doi: 10.1016/0092-8674(87)90499-5.


We examined the function of clathrin, a cytoplasmic protein associated with coated pits and vesicles, by introducing monoclonal antibodies into living cells and determining their effects on membrane transport. When anti-clathrin heavy chain antibodies were used, the following effects were observed: clathrin became aggregated in the cytoplasm, the number of coated pits on the plasma membrane was reduced, and adsorbtive endocytosis of Semliki Forest virus and fluid-phase endocytosis were decreased by 40%-50%. No change in transport of newly synthesized influenza hemagglutinin to the plasma membrane was observed. The results indicated that clathrin in CV-1 cells is involved in fluid-phase uptake and receptor-mediated endocytosis, but not in constitutive transport within the secretory pathway.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Monoclonal
  • Clathrin / immunology
  • Clathrin / physiology*
  • Coated Pits, Cell-Membrane / physiology
  • Endocytosis*
  • Endoplasmic Reticulum / metabolism
  • Fluorescent Antibody Technique
  • Hemagglutinins, Viral / metabolism
  • Membrane Proteins / metabolism
  • Semliki forest virus / immunology


  • Antibodies, Monoclonal
  • Clathrin
  • Hemagglutinins, Viral
  • Membrane Proteins