Stoichiometric quantification of spatially dense assemblies with qPAINT

Nanoscale. 2019 Jul 14;11(26):12460-12464. doi: 10.1039/c9nr00472f. Epub 2019 May 23.


Quantitative PAINT (qPAINT) is a useful method for counting well-separated molecules within nanoscale assemblies. But whether cross-reactivity in densely-packed arrangements perturbs measurements is unknown. Here we establish that qPAINT measurements are robust even when target molecules are separated by as little as 3 nm, sufficiently close that single-stranded DNA binding sites can interact.

MeSH terms

  • DNA, Single-Stranded / chemistry*
  • Nanotubes / chemistry*
  • Nanotubes / ultrastructure


  • DNA, Single-Stranded