Functional Fragments of AIMP1-Derived Peptide (AdP) and Optimized Hydrosol for Their Topical Deposition by Box-Behnken Design

Molecules. 2019 May 22;24(10):1967. doi: 10.3390/molecules24101967.


Aminoacyl-tRNA synthetase complex-interacting multifunctional protein 1 (AIMP1)-derived peptide (AdP) has been developed as a cosmeceutical ingredient for skin anti-aging given its fibroblast-activating (FA) and melanocyte-inhibiting (MI) functions. However, a suitable strategy for the topical delivery of AdP was required due to its low-permeable properties. In this study, FA and MI domains of AdP (FA-AdP and MI-AdP, respectively) were determined by functional domain mapping, where the activities of several fragments of AdP on fibroblast and melanocyte were tested, and a hydrosol-based topical delivery system for these AdP fragments was prepared. The excipient composition of the hydrosol was optimized to maximize the viscosity and drying rate by using Box-Behnken design. The artificial skin deposition of FA-AdP-loaded hydrosol was evaluated using Keshary-Chien diffusion cells equipped with Strat-M membrane (STM). The quantification of the fluorescent dye-tagged FA-AdP in STM was carried out by near-infrared fluorescence imaging. The optimized hydrosol showed 127-fold higher peptide deposition in STM than free FA-AdP (p < 0.05). This work suggests that FA- and MI-AdP are active-domains for anti-wrinkle and whitening activities, respectively, and the hydrosol could be used as a promising cosmetic formulation for the delivery of AdPs to the skin.

Keywords: AIMP1-derived peptide (AdP); Box-Behnken design; cosmeceutical peptide; hydrosol; topical delivery of peptides.

MeSH terms

  • Animals
  • Cell Line
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Cosmeceuticals / chemistry
  • Cosmeceuticals / pharmacology*
  • Cytokines / chemistry*
  • Doxorubicin
  • Humans
  • Melanocytes / cytology
  • Melanocytes / drug effects
  • Melanocytes / metabolism
  • Mice
  • Models, Biological
  • Neoplasm Proteins / chemistry*
  • Optical Imaging
  • Peptides / chemistry
  • Peptides / pharmacology*
  • RNA-Binding Proteins / chemistry*
  • Skin Aging / drug effects*
  • Viscosity


  • Cosmeceuticals
  • Cytokines
  • Neoplasm Proteins
  • Peptides
  • RNA-Binding Proteins
  • small inducible cytokine subfamily E, member 1
  • Doxorubicin