Salmonella Typhi, Paratyphi A, Enteritidis and Typhimurium core proteomes reveal differentially expressed proteins linked to the cell surface and pathogenicity

PLoS Negl Trop Dis. 2019 May 24;13(5):e0007416. doi: 10.1371/journal.pntd.0007416. eCollection 2019 May.

Abstract

Background: Salmonella enterica subsp. enterica contains more than 2,600 serovars of which four are of major medical relevance for humans. While the typhoidal serovars (Typhi and Paratyphi A) are human-restricted and cause enteric fever, non-typhoidal Salmonella serovars (Typhimurium and Enteritidis) have a broad host range and predominantly cause gastroenteritis.

Methodology/principle findings: We compared the core proteomes of Salmonella Typhi, Paratyphi A, Typhimurium and Enteritidis using contemporary proteomics. For each serovar, five clinical isolates (covering different geographical origins) and one reference strain were grown in vitro to the exponential phase. Levels of orthologous proteins quantified in all four serovars and within the typhoidal and non-typhoidal groups were compared and subjected to gene ontology term enrichment and inferred regulatory interactions. Differential expression of the core proteomes of the typhoidal serovars appears mainly related to cell surface components and, for the non-typhoidal serovars, to pathogenicity.

Conclusions/significance: Our comparative proteome analysis indicated differences in the expression of surface proteins between Salmonella Typhi and Paratyphi A, and in pathogenesis-related proteins between Salmonella Typhimurium and Enteritidis. Our findings may guide future development of novel diagnostics and vaccines, as well as understanding of disease progression.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Humans
  • Proteome / genetics*
  • Proteome / metabolism
  • Salmonella Infections / microbiology*
  • Salmonella enterica / genetics*
  • Salmonella enterica / metabolism
  • Salmonella enterica / pathogenicity
  • Salmonella paratyphi A / genetics*
  • Salmonella paratyphi A / metabolism
  • Salmonella paratyphi A / pathogenicity
  • Salmonella typhi / genetics*
  • Salmonella typhi / metabolism
  • Salmonella typhi / pathogenicity
  • Salmonella typhimurium / genetics*
  • Salmonella typhimurium / metabolism
  • Salmonella typhimurium / pathogenicity
  • Virulence

Substances

  • Bacterial Proteins
  • Proteome

Grant support

This work was supported by the Flemish Ministry of Sciences (EWI, SOFI project IDIS) (SD) and the InBev-Baillet Latour (IBL) (SD) Fund. The clinical isolates were obtained through the project “Surveillance of antimicrobial resistance among consecutive blood culture isolates in tropical settings”, that was funded by the Belgian Directorate of Development. Cooperation (DGD) (JJ) through the Third Framework Agreement between the Belgian DGD and the Institute of Tropical Medicine (ITM), Belgium. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.