The effects of Beeswax, Olive oil and Butter impregnated bandage on burn wound healing

Burns. 2019 Sep;45(6):1410-1417. doi: 10.1016/j.burns.2018.03.004. Epub 2019 May 22.


Background: Beeswax, Olive oil and Butter (BOB) are nutritive products that could support wound healing by adsorption to bandage. This study demonstrated the therapeutic effects of BOB on second degree burn.

Methods: Second degree burn model was created in rats. Experimental groups were assigned to Healthy, Burn, Silver Sulfadiazine (SS) and BOB. The effects of BOB were evaluated on skin regeneration, vesicles and bullae and fibroblast activity by histopathological analyses and wound contraction percent were determined. Transforming Growth Factor-Beta1 (TGF-β1) and Vascular Endothelial Growth Factor-alpha (VEGF-α) mRNA expressions were analyzed with Real Time-Polymerase Chain Reaction. All parameters analyzed at 3rd, 7th, 14th days.

Results: The BOB treatment increased TGF-β1 and VEGF-α expressions compared to Burn group. The histopathological analyses showed that epidermis and dermis layers injured due to burn. BOB treatment augmented the regeneration of these layers and increased fibroblast activity and keratinization which are play important role on the new blood vessels production. Also with the BOB treatment we showed wound contraction levels were higher than Burn and SS treatment.

Conclusion: This study demonstrated that beeswax-olive oil-butter mixture impregnated bandage treatment in a second-degree burn rat model improved burn wound healing and encouraged skin renewal via modulating tissue TGF-β1 and VEGF-α.

Keywords: Beeswax; Burn wound; Butter; Olive oil; Rat.

MeSH terms

  • Animals
  • Anti-Infective Agents, Local / pharmacology
  • Bandages*
  • Burns / genetics
  • Burns / metabolism
  • Burns / pathology
  • Burns / therapy*
  • Butter*
  • Fibroblasts / drug effects
  • Fibroblasts / pathology
  • Olive Oil / pharmacology*
  • RNA, Messenger / drug effects
  • RNA, Messenger / metabolism
  • Rats
  • Silver Sulfadiazine / pharmacology
  • Skin / drug effects*
  • Skin / metabolism
  • Skin / pathology
  • Transforming Growth Factor beta1 / drug effects
  • Transforming Growth Factor beta1 / genetics
  • Vascular Endothelial Growth Factor A / drug effects
  • Vascular Endothelial Growth Factor A / genetics
  • Waxes / pharmacology*
  • Wound Healing*


  • Anti-Infective Agents, Local
  • Olive Oil
  • RNA, Messenger
  • Tgfb1 protein, rat
  • Transforming Growth Factor beta1
  • Vascular Endothelial Growth Factor A
  • Waxes
  • vascular endothelial growth factor A, rat
  • beeswax
  • Butter
  • Silver Sulfadiazine