Hyaluronan/collagen hydrogel matrices containing high-sulfated hyaluronan microgels for regulating transforming growth factor-β1

J Mater Sci Mater Med. 2019 May 24;30(6):65. doi: 10.1007/s10856-019-6267-1.


Hyaluronan (HA)-based microgels generated in a microfluidic approach, containing an artificial extracellular matrix composed of collagen and high-sulfated hyaluronan (sHA3), were incorporated into a HA/collagen-based hydrogel matrix. This significantly enhanced the retention of noncrosslinked sHA3 within the gels enabling controlled sHA3 presentation. Gels containing sHA3 bound higher amounts of transforming growth factor-β1 (TGF-β1) compared to pure HA/collagen hydrogels. Moreover, the presence of sHA3-containing microgels improved the TGF-β1 retention within the hydrogels. These findings are promising for developing innovative biomaterials with adjustable sHA3 release and growth factor interaction profiles to foster skin repair, e.g., by rebalancing dysregulated TGF-β1 levels.

MeSH terms

  • Animals
  • Biocompatible Materials / chemistry
  • Cattle
  • Collagen / chemistry*
  • Extracellular Matrix / metabolism
  • Glycosaminoglycans / chemistry
  • Humans
  • Hyaluronic Acid / chemistry*
  • Hydrogels / chemistry*
  • Microfluidics
  • Microgels / chemistry*
  • Rats
  • Skin / metabolism
  • Skin / pathology
  • Streptococcus
  • Sulfates / metabolism
  • Transforming Growth Factor beta1 / metabolism*
  • Wound Healing


  • Biocompatible Materials
  • Glycosaminoglycans
  • Hydrogels
  • Microgels
  • Sulfates
  • TGFB1 protein, human
  • Transforming Growth Factor beta1
  • Hyaluronic Acid
  • Collagen