Reassessment of temperature-humidity index for measuring heat stress in crossbred dairy cattle of a sub-tropical region

Abstract

The temperature-humidity index (THI) has been extensively applied for assessing heat stress in moderate to hot conditions in dairy cattle. However, there exist wide variation between researchers in defining an appropriate range of THI values for denoting different levels of stress. The present study was aimed to reassess previously described heat stress indicators of dairy cattle of sub-tropical region of India. From comparative evaluation of meteorological data over previous four years (2014-2017) the period of year when high THI prevailed in the region was determined. Accordingly, the time period of sample collection and observation on animals was decided, so that a THI range of 68-86 could be covered. After analyzing physiological, biochemical parameters and expression profile of heat shock response (HSR) genes of animals in response to different THI, it was evident from the study that animal undergoes few or little changes at THI 72, but major physiological changes occurred after THI reached 74. At THI range 74-79, no drastic change in these parameters occurred suggesting animals undergo transient acclimatization in this range to maintain homeostasis. Once THI reached and crossed 80, this homeostasis was perturbed and animals experienced major physiological changes again. Overall, the study suggests that THI values indicating level of heat stress are dependent on the geographic location, as well as type of animal and therefore, existing THI should be recalibrated for different climatic region for accurate assessment of the heat stress.

Keywords: Heat stress; Hsf; Hsp70; Temperature humidity index; Thermal imaging.