Characterization, hypolipidemic and antioxidant activities of degraded polysaccharides from Ganoderma lucidum

Int J Biol Macromol. 2019 Aug 15:135:706-716. doi: 10.1016/j.ijbiomac.2019.05.166. Epub 2019 May 23.


The aim of this work is to characterize the primary structure and physicochemical properties of natural polysaccharides (GLP) and degraded polysaccharides (GLPUD) from Ganoderma lucidum, and evaluate their hypolipidemic and antioxidant activities. The results of particle size distribution and scanning electron microscopy (SEM) showed that Ganoderma lucidum polysaccharides were effectively degraded by ultrasonic method. GLPUD was composed of the same monosaccharide units as GLP but with different molar ratios. Infrared spectra and NMR showed that the primary structure of polysaccharides had not been changed by ultrasonic degradation. Meanwhile, the thermal stability of polysaccharides increased after ultrasonic treatment. After administration by GLP and GLPUD four weeks, body weight, visceral index, atherosclerosis index (AI) and biochemical indicators in serum and in liver were determined. The results showed that GLPUD had stronger hypolipidemic and antioxidant activities than GLP. GLPUD was more effective than the GLP for reducing AI, total cholesterol (TC), triglycerides (TG) and low-density lipoprotein cholesterol (LDL-C), raising high density lipoprotein (HDL-C) (p < 0.01), reducing malondialdehyde (MDA) content, as well as increasing the glutathione peroxidase (GSH-Px) in mice serum, increasing superoxide dismutase (SOD) activity and reducing MDA content in liver (p < 0.05 or p < 0.01). In addition, the histopathological observations of mice livers showed that GLPUD could significantly improve lipid metabolism disorder in hepatocytes. Thus, GLPUD might be tested as a more effective hypolipidemic drug.

Keywords: Antioxidant; Degradation; Ganoderma lucidum polysaccharides; Hypolipidemic.

MeSH terms

  • Animals
  • Antioxidants / chemistry*
  • Antioxidants / pharmacology*
  • Body Weight / drug effects
  • Chemical Phenomena
  • Cholesterol, HDL / blood
  • Cholesterol, LDL / blood
  • Fungal Polysaccharides / chemistry*
  • Fungal Polysaccharides / pharmacology*
  • Ganoderma / chemistry*
  • Glutathione Peroxidase / blood
  • Glutathione Peroxidase / metabolism
  • Hypolipidemic Agents / chemistry*
  • Hypolipidemic Agents / pharmacology*
  • Liver / drug effects
  • Liver / metabolism
  • Malondialdehyde / blood
  • Malondialdehyde / metabolism
  • Mice
  • Monosaccharides / analysis
  • Superoxide Dismutase / blood
  • Superoxide Dismutase / metabolism
  • Triglycerides / blood
  • Ultrasonic Waves


  • Antioxidants
  • Cholesterol, HDL
  • Cholesterol, LDL
  • Fungal Polysaccharides
  • Hypolipidemic Agents
  • Monosaccharides
  • Triglycerides
  • Malondialdehyde
  • Glutathione Peroxidase
  • Superoxide Dismutase