Mapping the Whole-Body Muscle Activity of Hydra vulgaris

Abstract

Hydra is a cnidarian polyp with an anatomically simple neuromuscular system that can offer evolutionary insights on the functional design of animal body plans. Using calcium imaging to map the activity of the entire epitheliomuscular system of behaving Hydra, we find seven basic spatiotemporal patterns of muscle activity. Patterns include global and local activation events with widely varying kinetics of initiation and wave-like propagation. The orthogonally oriented endodermal and ectodermal muscle fibers are jointly activated during longitudinal contractions. Individual epitheliomuscular cells can participate in multiple patterns, even with very different kinetics. This cellular multifunctionality could enable the structurally simple epitheliomuscular tissue of basal metazoans to implement a diverse behavioral output.

Keywords: Cnidaria; Hydra; animal evolution; basal metazoa; calcium imaging; emergence; epithelia; muscle; neural evolution; neuromuscular systems.