Highly efficient transgene-free targeted mutagenesis and single-stranded oligodeoxynucleotide-mediated precise knock-in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins

Plant Biotechnol J. 2019 Nov;17(11):2032-2034. doi: 10.1111/pbi.13174. Epub 2019 Jun 11.
No abstract available

Publication types

  • Editorial

MeSH terms

  • CRISPR-Cas Systems*
  • Euglena gracilis / growth & development*
  • Gene Editing*
  • Gene Knock-In Techniques
  • Microalgae / genetics*
  • Mutagenesis*
  • Oligodeoxyribonucleotides
  • Ribonucleoproteins

Substances

  • Oligodeoxyribonucleotides
  • Ribonucleoproteins