Illumination of cell cycle progression by multi-fluorescent sensing system

Cell Cycle. 2019 Jun;18(12):1364-1378. doi: 10.1080/15384101.2019.1618117. Epub 2019 May 26.

Abstract

Multi-fluorescent imaging of cell cycle progression is essential for the study of cell proliferation in vitro and in vivo. However, there remain challenges, particularly to image cell cycle progression in living cell with available imaging techniques due to lacking the suitable probe. Here, we design a triple fluorescent sensors system making the cell cycle progression visible. Multi-fluorescent sensor shows the proliferating or proliferated cells with different colors. We thus generate the construct and adenovirus to probe cell cycle progression in living cell lines and primary cardiomyocytes. Furthermore, we create the knock-in transgenic mouse to monitor cell cycle progression in vivo. Together, the system can be applied to investigate cell proliferation or cell cycle progression in living cells and animals.

Keywords: Cell cycle; EGFP; cyclin B1; imaging; multi-fluorescent sensors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Cycle* / genetics
  • Cell Line
  • Cyclin B1 / genetics
  • Fluorescence
  • Fluorescent Dyes / metabolism*
  • Green Fluorescent Proteins / metabolism
  • Humans
  • Mice, Transgenic
  • Mitosis / genetics
  • Promoter Regions, Genetic / genetics

Substances

  • Cyclin B1
  • Fluorescent Dyes
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins

Grants and funding

This work article was supported by the Natural Science Foundation of Tianjin, China (16JCYBJC43200), NSFC General Project (81773120), the Fundamental Research Funds for the Central Universities, Nankai University (63191214) to SY.