Isothermal Amplification Methods for the SNP Genotyping

Abstract

The demands for genotyping techniques with acceptable precision, accuracy, cost-effectiveness in high throughput formats made driving forces for continuous development of novel technologies. A wide range of mutation detection techniques based on polymerase chain reaction (PCR) have been introduced. The best alternatives were the isothermal amplification technologies that those did not require a thermal cycler. In this review, we aimed to describe the most known isothermal amplification techniques for SNP genotyping.

Keywords: Genotyping; Isothermal amplification; LAMP; Mutation detection; Polymerase chain reaction; SNP..