Cytological and transcriptome analysis reveal that interaction at Sb pollen sterility locus cause down-regulation of important meiosis-related genes associated with high pollen sterility in autotetraploid rice hybrids

Jinwen Wu; Muhammad Qasim Shahid; Minyi Chen; Xiang Li; Jirui Li; Xiaosong Xu; Susu Du; Xiangdong Liu

doi:10.1016/j.plaphy.2019.05.019

Cytological and transcriptome analysis reveal that interaction at Sb pollen sterility locus cause down-regulation of important meiosis-related genes associated with high pollen sterility in autotetraploid rice hybrids

Plant Physiol Biochem. 2019 Aug:141:73-82. doi: 10.1016/j.plaphy.2019.05.019. Epub 2019 May 21.

Authors

Jinwen Wu¹, Muhammad Qasim Shahid¹, Minyi Chen², Xiang Li¹, Jirui Li², Xiaosong Xu², Susu Du¹, Xiangdong Liu³

Affiliations

¹ State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China; Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China; College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.
² Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China; College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.
³ State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China; Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China; College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address: xdliu@scau.edu.cn.

PMID: 31132695
DOI: 10.1016/j.plaphy.2019.05.019

Abstract

Polyploidy could increase the interactions of pollen sterility loci and Sb locus interaction cause higher pollen abortion than other loci. Therefore, we focused on the interaction at Sb pollen sterility locus in autotetraploid rice compared to diploid rice hybrid using the near-isogenic lines in the present study. Cytological observations indicated that interaction at Sb locus cause high pollen sterility (69.9%) and abnormal chromosome behavior (37.02%) at Metaphase II in autotetraploid rice hybrid. A total of 139 meiosis-related or meiosis stage-specific genes were detected in the autotetraploid rice hybrid harboring interaction at Sb locus and 27 of these meiosis-related or specific genes displayed significant down-regulation, including four pollen fertility related genes (Rad51, XRI1, PSS1 and MIL1). These results revealed a stronger interaction at Sb pollen sterility locus than other loci, which cause down-regulation of many important meiosis-related genes that were associated with higher pollen sterility in autotetraploid rice hybrids.

Keywords: Autotetraploid rice; Meiosis; Pollen sterility; Polyploidy; Transcriptome analysis.

MeSH terms

Alleles
Cell Biology
Chromosomes, Plant
Crosses, Genetic*
Down-Regulation
Gene Expression Profiling
Gene Expression Regulation, Plant
Genotype
Heterozygote
Meiosis / genetics*
Oryza / genetics*
Plant Infertility / genetics*
Pollen / genetics*
Tetraploidy
Transcriptome