Light induces a rapid and transient increase in inositol-trisphosphate in toad rod outer segments

Biochem Biophys Res Commun. 1987 Aug 14;146(3):1392-6. doi: 10.1016/0006-291x(87)90804-7.

Abstract

The sub-second time course of changes in the content of [3H]inositol-1,4,5-trisphosphate was determined in rod outer segments from very rapidly frozen Bufo retinas that had been incubated with [3H]inositol. Rod outer segments were cut off frozen specimens with a cryostat microtome and the water soluble extracts were analyzed. The content of [3H]inositol-1,4,5-trisphosphate rose after approximately 250 msec of bright illumination, but returned to the unstimulated level after 1 sec, whether the stimulus remained on or not. That is, there was rapid but transient change in the content of [3H]inositol-1,4,5-trisphosphate after the onset of stimulation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bufo marinus
  • Darkness
  • Inositol 1,4,5-Trisphosphate
  • Inositol Phosphates / metabolism*
  • Kinetics
  • Light
  • Photoreceptor Cells / metabolism*
  • Rod Cell Outer Segment / metabolism*
  • Rod Cell Outer Segment / radiation effects
  • Sugar Phosphates / metabolism*

Substances

  • Inositol Phosphates
  • Sugar Phosphates
  • Inositol 1,4,5-Trisphosphate