Brief Report: Cell Cycle Dynamics of Human Pluripotent Stem Cells Primed for Differentiation

Stem Cells. 2019 Sep;37(9):1151-1157. doi: 10.1002/stem.3041. Epub 2019 Jun 10.


Understanding the molecular properties of the cell cycle of human pluripotent stem cells (hPSCs) is critical for effectively promoting differentiation. Here, we use the Fluorescence Ubiquitin Cell Cycle Indicator system adapted into hPSCs and perform RNA sequencing on cell cycle sorted hPSCs primed and unprimed for differentiation. Gene expression patterns of signaling factors and developmental regulators change in a cell cycle-specific manner in cells primed for differentiation without altering genes associated with pluripotency. Furthermore, we identify an important role for PI3K signaling in regulating the early transitory states of hPSCs toward differentiation. Stem Cells 2019;37:1151-1157.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle / genetics*
  • Cell Differentiation / genetics*
  • Cells, Cultured
  • Gene Expression Profiling / methods*
  • Gene Regulatory Networks*
  • Humans
  • Phosphatidylinositol 3-Kinases / metabolism
  • Pluripotent Stem Cells / cytology
  • Pluripotent Stem Cells / metabolism*
  • Sequence Analysis, RNA / methods
  • Signal Transduction