Accelerated wound healing process in rat by probiotic Lactobacillus reuteri derived ointment

J Basic Clin Physiol Pharmacol. 2019 May 28;30(3):/j/jbcpp.2019.30.issue-3/jbcpp-2018-0150/jbcpp-2018-0150.xml. doi: 10.1515/jbcpp-2018-0150.


Background Antioxidant agents have positive effects on wound healing process and on the other hand probiotics also have great antioxidation or oxidation-resistance properties. Methods In this study, we investigated the effects of Lactobacillus reuteri derived ointment as a probiotic bacterium on the wound healing process using a full-thickness rat skin model. The wound diameter and contraction percent measurement, histopathological examination, and myeloperoxidase (MPO) enzyme activity evaluation were carried out during the period of study. Results Amount of deposit collagen was significantly increased; epithelization speed and epidermis production were also observed in the ointment-treated group, while the inflammation rate was also significantly lower than the control group. In addition, a significant reduction in the activity of enzyme MPO was also detected in the ointment-treated group. Conclusions According to the results, probiotic ointment effectively accelerates the wound healing process and it can be used as a promising drug for wound dressing application.

Keywords: Lactobacillus reuteris; histopathology; myeloperoxidase (MPO); probiotics; wound healing.

MeSH terms

  • Animals
  • Disease Models, Animal
  • Lactobacillus reuteri*
  • Male
  • Ointments
  • Probiotics / administration & dosage
  • Probiotics / therapeutic use*
  • Rats, Sprague-Dawley
  • Skin / drug effects
  • Skin / injuries*
  • Skin / pathology
  • Wound Healing / drug effects*
  • Wounds, Penetrating / drug therapy*
  • Wounds, Penetrating / pathology


  • Ointments