miR-153-3p Suppresses Inhibitor of Growth Protein 2 Expression to Function as Tumor Suppressor in Acute Lymphoblastic Leukemia

Technol Cancer Res Treat. 2019 Jan-Dec;18:1533033819852990. doi: 10.1177/1533033819852990.

Abstract

Alterations in microRNAs expression can accelerate the development of human cancers. However, the role of miR-153-3p in acute lymphoblastic leukemia remains unknown. The expression of miR-153-3p in acute lymphoblastic leukemia cell lines was measured by quantitative real-time polymerase chain reaction. Effects of miR-153-3p expression on acute lymphoblastic leukemia cell proliferation, migration, and invasion were examined by Cell Counting Kit-8 assay, wound healing assay, and Transwell invasion assay, respectively. We then validated inhibitor of growth protein 2 as a direct target of miR-153-3p through bioinformatics analysis, luciferase activity reporter assay, and Western blot assay. The miR-153-3p expression was decreased in acute lymphoblastic leukemia cell lines. Cell proliferation, migration, and invasion of acute lymphoblastic leukemia were obviously decreased by miR-153-3p overexpression. Moreover, inhibitor of growth protein 2 was validated as a direct target of miR-153-3p and the overexpression of inhibitor of growth protein 2 reversed the suppressive effects of miR-153-3p on acute lymphoblastic leukemia cell behaviors. Based on these results, we provided evidence that miR-153-3p might be a target for the treatment of acute lymphoblastic leukemia.

Keywords: ING2; acute lymphoblastic leukemia; cell behaviors; miR-153-3p; tumor suppressor.

Publication types

  • Retracted Publication

MeSH terms

  • 3' Untranslated Regions
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation
  • Gene Expression Regulation, Neoplastic*
  • Genes, Tumor Suppressor*
  • Homeodomain Proteins / genetics*
  • Humans
  • MicroRNAs / genetics*
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / genetics*
  • RNA Interference*
  • Receptors, Cytoplasmic and Nuclear / genetics*
  • Tumor Suppressor Proteins / genetics*

Substances

  • 3' Untranslated Regions
  • Homeodomain Proteins
  • ING2 protein, human
  • MIRN153 microRNA, human
  • MicroRNAs
  • Receptors, Cytoplasmic and Nuclear
  • Tumor Suppressor Proteins