RNA methyltransferase BCDIN3D is crucial for female fertility and miRNA and mRNA profiles in Drosophila ovaries

PLoS One. 2019 May 30;14(5):e0217603. doi: 10.1371/journal.pone.0217603. eCollection 2019.


RNA methyltransferases post-transcriptionally add methyl groups to RNAs, which can regulate their fates and functions. Human BCDIN3D (Bicoid interacting 3 domain containing RNA methyltransferase) has been reported to specifically methylate the 5'-monophosphates of pre-miR-145 and cytoplasmic tRNAHis. Methylation of the 5'-monophosphate of pre-miR-145 blocks its cleavage by the miRNA generating enzyme Dicer, preventing generation of miR-145. Elevated expression of BCDIN3D has been associated with poor prognosis in breast cancer. However, the biological functions of BCDIN3D and its orthologs remain unknown. Here we studied the biological and molecular functions of CG1239, a Drosophila ortholog of BCDIN3D. We found that ovary-specific knockdown of Drosophila BCDIN3D causes female sterility. High-throughput sequencing revealed that miRNA and mRNA profiles are dysregulated in BCDIN3D knockdown ovaries. Pathway analysis showed that many of the dysregulated genes are involved in metabolic processes, ribonucleoprotein complex regulation, and translational control. Our results reveal BCDIN3D's biological role in female fertility and its molecular role in defining miRNA and mRNA profiles in ovaries.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • DNA-Binding Proteins / genetics
  • Drosophila Proteins / genetics*
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / growth & development
  • Female
  • Fertility / genetics*
  • Gene Expression Regulation, Developmental / genetics
  • Metabolism / genetics
  • Methylation
  • Methyltransferases / genetics*
  • MicroRNAs / genetics
  • Ovary / growth & development*
  • Ovary / metabolism
  • Protein O-Methyltransferase / genetics*
  • RNA, Messenger / genetics
  • Ribonuclease III / genetics


  • DNA-Binding Proteins
  • Drosophila Proteins
  • MicroRNAs
  • RNA, Messenger
  • CG1239 protein, Drosophila
  • Methyltransferases
  • Protein O-Methyltransferase
  • Ribonuclease III