Lactobacillus rhamnosus GG microcapsules inhibit Escherichia coli biofilm formation in coculture

Biotechnol Lett. 2019 Sep;41(8-9):1007-1014. doi: 10.1007/s10529-019-02694-2. Epub 2019 May 30.


Objectives: Microbial biofilms have become one of the most significant causes of nosocomial infections. The aim of this study was to examine the potential quorum sensing inhibitor activities of Lactobacillus rhamnosus GG microcapsules.

Results: Lactobacillus rhamnosus GG microcapsules effectively inhibited initial biofilm formation at a concentration of 2.5 × 108 CFU/mL. Furthermore, the inhibition rate was increased to 79% in the Lactobacillus rhamnosus GG microcapsules group, resulting in a reduction in the biofilm maturation stage. In addition, real-time PCR analysis revealed that the LGG microcapsules can act as effective inhibitors of transcriptional activators of the quorum sensing circuit in E.coli, luxS, lsrK, and lsrR.

Conclusions: Lactobacillus rhamnosus GG microcapsules can effectively inhibit biofilm formation and disturb mature biofilms.

Keywords: Biofilm inhibition; Coculture model; Probiotic microcapsules; Quorum sensing.

MeSH terms

  • Antibiosis*
  • Biofilms / growth & development*
  • Capsules
  • Coculture Techniques
  • Escherichia coli / growth & development*
  • Lactobacillus rhamnosus / growth & development*


  • Capsules