Abstract
The cohesin complex is required to establish sister chromatid cohesion and ensure accurate chromosome segregation after DNA replication. Recent data has also revealed a role for cohesion as a major player in DNA repair and gene expression regulation. All subunits of the Cohesin complex are essential and cannot be deleted. Here, we describe a protocol to efficiently deplete cohesin subunits with an auxin-inducible degron (AID) system in S. cerevisiae.
Keywords:
Auxin degron; Cohesin; S. cerevisiae.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Cycle Proteins / metabolism*
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Chromatids / metabolism
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Chromosomal Proteins, Non-Histone / metabolism*
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Chromosome Segregation / drug effects
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Chromosome Segregation / physiology
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Cohesins
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DNA Repair / drug effects
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DNA Repair / physiology
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DNA Replication / drug effects
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DNA Replication / physiology
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Indoleacetic Acids / pharmacology*
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Proteolysis / drug effects*
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Saccharomyces cerevisiae / drug effects*
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins / metabolism*
Substances
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Cell Cycle Proteins
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Chromosomal Proteins, Non-Histone
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Indoleacetic Acids
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Saccharomyces cerevisiae Proteins