Degradation of S. cerevisiae Cohesin with the Auxin-Inducible Degron System

Methods Mol Biol. 2019:2004:17-24. doi: 10.1007/978-1-4939-9520-2_2.

Abstract

The cohesin complex is required to establish sister chromatid cohesion and ensure accurate chromosome segregation after DNA replication. Recent data has also revealed a role for cohesion as a major player in DNA repair and gene expression regulation. All subunits of the Cohesin complex are essential and cannot be deleted. Here, we describe a protocol to efficiently deplete cohesin subunits with an auxin-inducible degron (AID) system in S. cerevisiae.

Keywords: Auxin degron; Cohesin; S. cerevisiae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle Proteins / metabolism*
  • Chromatids / metabolism
  • Chromosomal Proteins, Non-Histone / metabolism*
  • Chromosome Segregation / drug effects
  • Chromosome Segregation / physiology
  • Cohesins
  • DNA Repair / drug effects
  • DNA Repair / physiology
  • DNA Replication / drug effects
  • DNA Replication / physiology
  • Indoleacetic Acids / pharmacology*
  • Proteolysis / drug effects*
  • Saccharomyces cerevisiae / drug effects*
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / metabolism*

Substances

  • Cell Cycle Proteins
  • Chromosomal Proteins, Non-Histone
  • Indoleacetic Acids
  • Saccharomyces cerevisiae Proteins