Box-Behnken design based multi-objective optimisation of sequential extraction of pectin and anthocyanins from mango peels

Carbohydr Polym. 2019 Sep 1:219:29-38. doi: 10.1016/j.carbpol.2019.05.015. Epub 2019 May 8.

Abstract

Multi-objective sequential fractionation of mango (Mangifera indica L.) peels for pectin and anthocyanins in a biorefinery, was investigated with the aims of enhancing pectin purity, bioactivity and increasing recovered products. Anthocyanins were extracted from mango peels based on a Box-Behnken experimental design (ethanol concentration (50-80%), acetic acid concentration (0.1-2%), time (60-150 min) and temperature (25 °C)). The remaining residue was subsequently fractionated to recover pectin. The optimum anthocyanin conditions (67.45% ethanol, 0.24% acetic acid and 60 min) resulted in pectin with a 5.5% higher antioxidant activity, it was 23% richer in polygalacturonic acid (PGA) and had a yield of 31% w/w higher than conventionally extracted pectin. An anthocyanin extract with anthocyanin contents, polyphenol contents, and antioxidant activity of 22.33 mg/100 g, 132.62 mg/g, and 1.36 μg gallic acid equivalent, respectively, was obtained. Therefore, the sequential extraction of anthocyanins and pectin improves pectin functional properties and increases the mango peel value potential.

Keywords: Anthocyanin recovery; Biorefinery; Box-Behnken design; Mango peels; Pectin recovery; Sequential extraction.

MeSH terms

  • Anthocyanins* / chemistry
  • Anthocyanins* / isolation & purification
  • Chemical Fractionation / methods*
  • Fruit / chemistry*
  • Mangifera / chemistry*
  • Pectins* / chemistry
  • Pectins* / isolation & purification
  • Research Design

Substances

  • Anthocyanins
  • Pectins