Improving biocatalysis of cefaclor with penicillin acylase immobilized on magnetic nanocrystalline cellulose in deep eutectic solvent based co-solvent

Xiaoling Wu; Jun Xiong; Zixuan Huang; Shilin Cao; Minhua Zong; Wenyong Lou

doi:10.1016/j.biortech.2019.121548

Improving biocatalysis of cefaclor with penicillin acylase immobilized on magnetic nanocrystalline cellulose in deep eutectic solvent based co-solvent

Bioresour Technol. 2019 Sep:288:121548. doi: 10.1016/j.biortech.2019.121548. Epub 2019 May 24.

Authors

Xiaoling Wu¹, Jun Xiong¹, Zixuan Huang¹, Shilin Cao², Minhua Zong¹, Wenyong Lou³

Affiliations

¹ Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, No. 381 Wushan Road, Guangzhou 510640, Guangdong, China.
² School of Food Science and Engineering, Foshan University, Foshan 528000, China.
³ Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, No. 381 Wushan Road, Guangzhou 510640, Guangdong, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, No. 381 Wushan Road, Guangzhou 510640, China. Electronic address: wylou@scut.edu.cn.

PMID: 31152956
DOI: 10.1016/j.biortech.2019.121548

Abstract

Deep eutectic solvent (DES), has been considered as a new type of green solvent applied in enzymatic systems. Here, we reported DES-buffer co-solvent as a novel reaction medium for high efficient synthesis of cefaclor by penicilin acylase immobilized on magnetic nanocrystalline cellulose. Effect of DES composition, DES-buffer ratio, temperature, pH, substrate ratio and substrate concentration was systematically investigated. In co-solvent consisting of choline chloride (ChCl):glycol-buffer (7:3, v/v), conversion of 7-ACCA was 94%, synthesis to hydrolysis ratio was 1.8, and yield of cefaclor reached 91%, higher than that in aqueous buffer with optimized yield of 84%, showing the great potential of DES as organic solvent alternative. To the best of our knowledge, this is the first example of biosynthesis of cefaclor in the DES-buffer co-solvent.

Keywords: Biosynthesis; Cefaclor; Deep eutectic solvent; Magnetic nanocrystalline cellulose; Penicilin acylase.

MeSH terms

Biocatalysis
Cefaclor*
Cellulose
Penicillin Amidase*
Solvents

Substances

Solvents
Cefaclor
Cellulose
Penicillin Amidase