Effect of low-level laser therapy and zoledronic acid on bone repair process

Lasers Med Sci. 2019 Aug;34(6):1081-1088. doi: 10.1007/s10103-019-02810-8. Epub 2019 Jun 1.


This study aimed to evaluate, through histomorphometric analysis, the bone repair process in the tibia of rats treated with zoledronic acid and submitted to 808-nm low-level laser therapy (LLLT) by using arsenide aluminum gallium laser. For this purpose, 20 rats were used and distributed according to treatment: group 1-saline administration; group 2-treated with LLLT; group 3-treated with zoledronic acid; and group 4-treated with zoledronic acid and LLLT. The zoledronic acid was administered at a dose of 0.035 mg/kg every 2 weeks for 8 weeks. Subsequently, bone defects of 2 mm were prepared in the tibias of all groups. The bone defects in groups 2 and 4 were irradiated with LLLT in the immediate post-operative period. After 14 and 28 days of application, the animals were submitted and euthanized for histomorphometric analysis. The results were submitted to statistical analysis (α = 5%), and the intragroup comparison was performed using the t test. On the other hand, for intergroup comparison, the ANOVA test was performed, and to the groups presenting statistically significant difference, the Student-Newman-Keuls test was used. In intergroup comparison, group 1 (mean ± SD= 45.2 ± 18.56%) showed a lower bone formation compared with groups 2 (64.13 ± 3.51%) (p = 0.358) and 4 (15.2 ± 78.22%) (p = 0.049), at the 14-day period. Group 3 (20.99 ± 7.42%) also presented a lower amount of neoformed bone tissue, with statistically significant difference when compared with groups 1 (p = 0.002), 2, and 4 (p ≤ 0,001). After 28 days, group 1 presented a lower amount of neoformed bone tissue compared with the other groups, with p = 0.020. Thus, it was concluded that LLLT associated with zoledronic acid is effective for stimulating bone formation in surgically created defects in rats, at the periods studied.

Keywords: Bisphosphonate; Bone regeneration; Laser therapy; Rats.

MeSH terms

  • Animals
  • Female
  • Lasers, Semiconductor
  • Low-Level Light Therapy*
  • Osteogenesis / drug effects
  • Osteogenesis / radiation effects
  • Osteotomy
  • Rats, Wistar
  • Tibia / drug effects*
  • Tibia / pathology
  • Tibia / radiation effects*
  • Wound Healing / drug effects*
  • Wound Healing / radiation effects*
  • Zoledronic Acid / pharmacology*


  • Zoledronic Acid