Expression of the guanine nucleotide exchange factor, RAPGEF5, during mouse and human embryogenesis

Gene Expr Patterns. 2019 Dec;34:119057. doi: 10.1016/j.gep.2019.119057. Epub 2019 Jun 1.


Rap GTPases mediate fundamental cellular processes, including cell adhesion, migration and intracellular signal transduction. The subcellular activity of these GTPases is regulated by dedicated activators (guanine nucleotide exchange factors, GEFs) and deactivators (GTPase-activating proteins, GAPs). RAPGEF5 is a potent activator of Rap proteins and mutations in RAPGEF5 have been linked to both neurological disorders and congenital heart disease. In the frog model, Xenopus tropicalis, Rapgef5 is a critical regulator of the canonical Wnt signalling pathway and is required for normal gastrulation and correct establishment of the left-right body axis. However, requirements for RAPGEF5 in other developmental contexts, and in mammalian embryogenesis in particular, remain undefined. Here, we describe RAPGEF5 mRNA expression patterns during mouse (E9.5 - E16.5) and human (Carnegie stage 21) development, as an initial step towards better understanding its developmental functions.

Keywords: Guanine nucleotide exchange factor; RAP GTPase; Rapgef5; mRNA expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Embryo, Mammalian / metabolism
  • Embryonic Development / genetics
  • GTPase-Activating Proteins
  • Gene Expression Regulation, Developmental / genetics
  • Guanine Nucleotide Exchange Factors / genetics
  • Guanine Nucleotide Exchange Factors / metabolism
  • Humans
  • Mice / embryology
  • Sequence Homology, Amino Acid
  • Wnt Signaling Pathway
  • ras Guanine Nucleotide Exchange Factors / genetics*
  • ras Guanine Nucleotide Exchange Factors / metabolism


  • GTPase-Activating Proteins
  • Guanine Nucleotide Exchange Factors
  • RAPGEF5 protein, human
  • ras Guanine Nucleotide Exchange Factors